The result of pH to the kinetic constants for your hydrolysis of substrates by NagA was calculated within an attempt to discover All those purposeful groups while in the substrate and Energetic web site that happen to be necessary to be in a certain condition of protonation. The log k
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with several different substrate analogs. The response mechanism is of interest since NagA from bacterial sources is discovered with possibly one or two divalent steel ions while in the Lively web page.
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can bind up to 1 divalent cation to the activation of your hydrolytic h2o molecule. This drinking water molecule is additional activated by standard base catalysis throughout the abstraction of a proton by Asp-273.